Salivary and urinary profiles of melatonin and cortisol

Melatonin and cortisol saliva assays allow for the analysis of temporal variations and circadian rhythm of these hormones, while urine assays provide an integrated measure of their overall 24-hour production.

These profiles help characterise delayed or advanced sleep phase, irregular rhythms, and selected endocrine contributions to sleep disruption — guiding chronotherapy, light timing, and specialist referral when needed.

• Saliva samples at scheduled times.
• Urine collections for integrated 24-hour hormone production.
• Strict timing instructions for valid phase estimates.
• Interpretation by clinicians familiar with circadian biology.

The results are combined with actigraphy, sleep diaries, and clinical assessment to personalize light exposure, sleep scheduling, and medication timing.